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Abnova™ Fluorescent Dye 647-I Streptavidin
Fluorescence Dye 647-I with Streptavidin conjugate
Brand: Abnova™ U0297.200ug
Additional Details : Weight : 0.00010kg
Description
The slight red shift in absorption spectrum makes Fluorescence Dye 647-I an optimal match to filters designed Cy5 dyes. Besides, their fluorescence is pH-independent from pH 3 to 11. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Spectral Equivalent: Alexa Fluor 647, Cy5, DyLight 650
- Molar Extinction Co-efficient: ≥2,40,000 M-1cm-1
- Excitation (Max): 649nm
- Emission (Max): 664nm
- Solubility: Water
- Storage Buffer: Water
Application: Conjugation, Flow Cytometry
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
| Fluorescence Dye 647-I with Streptavidin conjugate. The slight red shift in absorption spectrum makes Fluorescence Dye 647-I an optimal match to filters designed Cy5 dyes.Besides, their fluorescence is pH-independent from pH 3 to 11. | |
| 200 μg | |
| Alexa Fluor 647, Cy5, DyLight 650 | |
| Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. | |
| 664 |
| Conjugation; Flow Cytometry | |
| Fluorescent Dye 647-I Streptavidin | |
| Water | |
| Fluorescent Dye 647-I Streptavidin | |
| 649 |
For research use only (RUO).