Invitrogen™ MEGAscript™ T7 Transcription Kit

Description

The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.

Using the MEGAscript T7 Kit:
The MEGAscript T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3 to 5mg of RNA (approximately 100μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400 to 650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10 to 50 times the amount of RNA produced by conventional transcription reactions.

Applications:The MEGAscript T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all four nucleotides is high, well above the K_m for the enzyme. The MEGAscript T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.

Accessory products:
MessageAmp™ aRNA amplification kits are powered by MEGAscript technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, use MEGAclear™ Kit (Mfr. No. AM1908) and the MEGAclear-96 Kit (Mfr.. No. AM1909).

RNA Yield:
100 μg