Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody, Invitrogen™

Description

This antibody recognizes human IgG Fc (all subclasses). The epitope detected lies within the CH2 domain as determined by haemagglutination and western blotting using IgG heavy chain and myelomas with defined domain deletions. The epitope is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. MA5-16859 cross-reacts with Rhesus monkey.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.