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Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit
These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
Brand: Invitrogen™ EEA019
Additional Details : Weight : 0.40000kg
Description
The Reactive Oxygen Species (ROS) Fluorometric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ROS in fresh tissue and live cell samples. ROS are highly reactive molecules that play a crucial role in various physiological and pathological processes, including oxidative stress, inflammation, and cellular signaling.
The assay works by utilizing the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) to detect the presence of ROS. DCFH-DA is taken up by cells and is converted to dichlorofluorescein (DCFH) by intracellular esterase. DCFH is oxidized by ROS to the strong fluorescent DCF. The fluorescence of DCF (Ex/Em 502/525 nm) is directly proportional to the amount of ROS present in the sample.
Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results
Specifications
| Reagent 1: –20°C Reagent 2: 2–8°C |
|
| Cell Culture, Live Tissue | |
| 30-60 min. | |
| Reactive Oxygen Species (ROS) |
| Fluorescence | |
| Spectrophotometer, Microplate Reader, Flow Cytometer | |
| Dry Ice, 2–8°C | |
| 60 min. |