Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780

Description

• Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
• Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
• Fixable Viability Dye eFluor™ 780 can be excited by the red (633 nm) laser line and has a peak emission of 780 nm that can be detected using a 780/60 band pass filter (equivalent to APC-eFluor™ 780 or APC-Alexa Fluor™ 750)
• For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
• If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
• After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
• Testing suggests that compensation out of most detectors is negligible, with compensation out of PE-Cyanine7 being the highest at less than 5%
• Actual compensation values will depend on each investigator's specific instrument, filter sets, and PMT voltage settings
• Fixable Viability Dye eFluor™ 780 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
• It should be protected from light and moisture
• Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
• Allow vial to equilibrate to room temperature before opening