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Thermo Scientific™ GeneJET Genomic DNA Purification Kit

Brand: Thermo Scientific™ K0722

5190.00 SEK valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10410450

6920.00 SEK / Each

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Description

Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.

The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.

Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes

Applications
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting

Specifications

Column Type	Spin Column
Isolation Technology	Silica Spin Column
Sample Type	Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Final Product Type	gDNA
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Shipping Condition	Room Temperature
Yield	≤30 μg

Content And Storage	• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening • 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening • 55 mL Digestion Solution; room temperature • 2 x 60 mL Lysis Solution; room temperature • 40 mL Wash Buffer I (concentrated); room temperature • 40 mL Wash Buffer II (concentrated); room temperature • 150 mL Elution Buffer; room temperature • 250 GeneJET Purification Columns with Collection Tubes; room temperature • 250 Collection Tubes; room temperature
Scale	Mini
Elution Volume	200 μL
For Use With (Application)	PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity	250 Preps
Test Time	20 min. (After sample homogenization)
Starting Material Amount	Bacteria: ≤10⁹ cells Cells: ≤10⁶ Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse) Tissue: ≤20 mg Whole Blood: ≤200 μL Yeast: ≤10⁸ cells

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

GeneJET Genomic DNA Purification Kit

Signal Word

Danger

Hazard Category

Acute toxicity Category 4
LONG-TERM AQUATIC HAZARD Chronic 3
Serious eye damage/eye irritation Category 2
Respiratory sensitiser Category 1
Skin corrosion/irritation Category 2

Hazard Statement

H302-Harmful if swallowed.
H315-Causes skin irritation.
H319-Causes serious eye irritation.
H332-Harmful if inhaled.
H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P273-Avoid release to the environment.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P284-¢In case of inadequate ventilation! wear respiratory protection.
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P330-Rinse mouth.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Proteinase, tritirachium album serine, glycerine, Sodium lauryl sulfate, Guanidine hydrochloride

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Thermo Scientific™ GeneJET Genomic DNA Purification Kit

Description

Specifications

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Safety and Handling

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