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Gibco™ DMEM/F-12, GlutaMAX™ supplement
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DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 10565018

433.49 SEK valid until 2024-12-31
Use promo code "21883" to get your promotional price.


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Product Code. 11559726

  • 480.00 SEK / 500mL

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For Research Use Only. All usage must comply with product instructions.


Requires : Supplementation
This item is not returnable. View return policy

Description

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With: GlutaMAX, Phenol Red

Without: HEPES

The complete formulation is available.

Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Animal Origin-free
Liquid
Standard Serum Supplementation
Sterile-filtered
No HEPES
Gibco™, GlutaMAX™
Room Temperature
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
1 X
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterile-filtered
High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
500 mL
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.