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Gibco™ MEM, powder
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Specifications
| Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
|---|---|
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Form | Powder |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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11534516
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Gibco™
61100087 |
10 L |
N/A
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N/A | |||||
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11544516
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Gibco™
61100103 |
50 L |
N/A
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N/A | |||||
Description
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
The complete formulation is available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.
Using MEM
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
|
| Primary Rat Astrocytes | |
| Powder | |
| Standard Serum Supplementation | |
| Low Glucose, Glutamine, Phenol Red | |
| cGMP-compliant under the ISO 13485 standard | |
| Room Temperature |
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| Animal Origin-free | |
| MEM (Minimum Essential Medium) | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate | |
| Gibco™ |
Safety and Handling
missing translation for 'shelfLife' : 36 Months From Date of Manufacture
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.