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Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
Brand: Cytiva 17-0717-01
Includes: Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems
Description
Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH
- Designed for use with a syringe, peristaltic pump, or chromatography system
- Degree of substitution: 10 μmol NHS/mL medium
- Delivered in 100% isopropanol to protect the active groups
- Stable in the pH range 3 to 12, depending upon ligand
- Average particle size: 34μm
Specifications
| 1 x 5mL | |
| 34 μm | |
| 5mL | |
| 1.6cm | |
| 0.98 in. | |
| 10μM NHS groups/mL medium | |
| 42psi | |
| 6% Highly Cross-linked Agarose |
| 20 mL/min. | |
| Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system | |
| NHS-Activated HP Column | |
| Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines | |
| 2.5cm | |
| Polypropylene | |
| 3 to 12 | |
| 4° to 8°C |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 2
- Serious eye damage/eye irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : propan-2-ol