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Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ 411002
Additional Details : Weight : 0.10000kg
Description
The Human Interferon alpha A, 2, A/D, D, K and 4b (Hu IFNαA, IFNα2, IFNαA/D, IFNαD, IFNαK and IFNα4b ) ELISA quantitates Hu IFNα in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNα. Principle of the method The Human IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Exhibits antiviral activity against Theiler's virus, Mengo virus and vesicular stomatitis virus. Interferons alpha stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase.Specifications
| P01562, P01563, P05013, P05014 | |
| Human IFN alpha ELISA Kit (Supernatant) | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| Supernatant | |
| 5 x 96 microplate wells | |
| Colorimetric Microplate Reader | |
| <8% | |
| Pre-coated 96 well Microplate, Detection Antibody Concentrate, Anti-IgG-HRP Concentrate, Concentrate Diluent, Standard, Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Adhesive Plate Covers | |
| Absorbance at 450nm | |
| 5 x 96 Tests | |
| Supernatant, 100 μL | |
| Human | |
| 3 hr. 15 min. |
| 12.5-500 pg/ml or 156-5000 pg/ml | |
| HRP | |
| ELISA Kit | |
| No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß or IFN-g; bovine IFN-t | |
| Human | |
| IFL,IFN,IFN-ALPHA,IFN-alphaD3,IFNA@,IFNA1,IFN-alphaA,IFNAB,INFA2,IFNA2 | |
| <8% | |
| HRP | |
| Intra-assay CV ≤ 8%; inter-assay CV ≤ 8% | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |