Invitrogen™ Human IL-8 (CXCL8) (1-77), Recombinant Protein

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. Description: Human IL-8, also called neutrophil attractant/activating protein (NAP-1), monocyte derived neutrophil activating peptide (MONAP), monocyte derived neutrophil activating factor (ANF), monocyte derived neutrophil chemotactic factor (MDNCF), and neutophil activating factor (NAF), is a ∽6-8kDa factor produced by variety types of cells including monocytes, lymphocytes, granulocytes, fibroblasts and endothelial cells. IL-8 is an inflammatory cytokine which functions as a neutrophil chemoatractant and activating factor. It also attracts basophils and a subpopulation of lymphocytes. Applications Reported: Recombinant human IL-8 is biologically active. Applications Tested: This recombinant human IL-8 has been tested in bioassays for chemoattractant activity, with maximal activity between 10-100ng/ml. Source: E. coli expressed amino acids Ser 28-Ser 99 of human IL-8 accession # NM_000584. Bioactivity: Measured by chemotaxis assay of human peripheral blood neutrophils. Maximal chemoattractant activity was observed between 10-100ng/mL. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 8,386. The DTT reduced protein migrates as an 8kDa polypeptide and the non-reduced polypeptide migrates as a 9kDa polypeptide on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.