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Invitrogen™ DES™-Inducible/Secreted Kit with pCoHygro
For inducible, secreted expression of recombinant proteins and hygromycin (pCoHygro)
Brand: Invitrogen™ K413001
Includes: 20μg pCoHygro, pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2μg each), GIBCO Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent.
Description
- Offers inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride
- Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-ch
- N-terminal signal sequence from insect BiP gene is provided to direct recombinant fusion protein through secretory pathway of S2 cells into culture medium
- Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
- To facilitate cloning, set of three vectors, A, B and C is provided; Each vector has multiple cloning site in different reading frame relative to the BiP signal sequence
Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells
Specifications
| Copper Sulfate | |
| The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored. | |
| Restriction Enzyme/MCS | |
| pMT |
| Inducible/Secreted Kit | |
| His Tag (6x), V5 Epitope Tag | |
| 1 kit | |
| DES™ |
For Research Use Only. Not for use in diagnostic procedures.