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Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit
Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis
Brand: Invitrogen™ 11917020
Includes: Reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50μg total RNA or 0.2-5μg mRNA into double-stranded cDNA.
Requires : Primer
This item is not returnable. View return policy
Description
Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA).
Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.
- Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
- Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
- Optimal temperature: 42°C
DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Wet Ice
Specifications
| The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 μg total RNA or 0.2-5 μg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored. | |
| Direct Labeling | |
| SuperScript™ | |
| 50 Reactions | |
| Wet Ice | |
| cDNA (Double-Stranded) |
| Kit | |
| 42°C | |
| cDNA Synthesis Kit | |
| SuperScript™ II | |
| Microarray Analysis | |
| RNA |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
For Research Use Only. Not for use in diagnostic procedures.