missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ mCP Selective Agar
Isolate Clostridium perfringens in water with mCP Selective Agar and idenitfy characteristic growth of opaque yellow colonies.
Brand: Thermo Scientific™ PO5163A
324.45 SEK valid until 2025-01-31
Use promo code "23894" to get your promotional price.
This item is not returnable.
View return policy
Description
Isolate Clostridium perfringens in water with Thermo Scientific™ mCP Selective Agar and idenitfy characteristic growth of opaque yellow colonies. Most other Clostridium spp. will appear as either purple colonies, due to the lack of sucrose fermentation, or blue/green colonies where the organism is still cleaving Indoxyl β-D glucoside and also fermenting sucrose.
In mCP Medium, lack of b-D-glucosidase activity (an enzyme involved in cellobiose fermentation), fermentation of sucrose, and production of acid phosphatase are used to differentiate presumptive Clostridium perfringens colonies from other Clostridium spp. Lack of β-D glucosidase activity means that Clostridium perfringens does not cleave the chromogen, indoxyl β-D glucoside, in the medium. Furthermore, as the organisms ferment the sucrose in the medium, reducing the pH, bromocresol purple changes from purple to yellow. This results in characteristic opaque yellow Clostridium perfringens colonies. Most other Clostridium spp. will appear as either purple colonies, due to the lack of sucrose fermentation, or blue/green colonies where the organism is still cleaving Indoxyl b-D glucoside and also fermenting sucrose.Presumptive positive Clostridium perfringens colonies can be further tested for acid phosphatase activity by exposure to ammonium hydroxide vapour for 20 to 30 seconds. Clostridium perfringens colonies turn pink or red as phenolphthalein diphosphate is cleaved by acid phosphatase. No color change will be seen with colonies of organisms that do not posses acid phosphatase. It is important this further test is carried out as there are a very small number of non-perfringens clostridia that produce yellow colonies; however, these colonies will remain yellow after exposure to ammonium hydroxide as they are acid phosphatase-negative.
Not all products are available for sale in all territories. Please inquire.
Remel™ and Oxoid™ products are now part of the Thermo Scientific brand, combining powerful manual, semi-automated and fully automated test products and a comprehensive line of media and diagnostic products to offer a complete, end-to-end solution to quickly deliver the products you need and the quality results your laboratory depends on.
Specifications
Tested in accordance with CEN ISO/TS 11133-2. | |
6°C to 12°C | |
90mm Monoplate | |
Agar | |
Flow Wrapped - Single Layer |
Blue lilac, transparent | |
mCP Selective Agar | |
Germany | |
17g ± 5% | |
10/Pk. |