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Macherey-Nagel™ NucleoSpin™ RNA Clean-up Column
Recommended for the clean-up of total RNA from RNA preparations which contain inacceptable amounts of RT-PCR* inhibitors
Brand: Macherey-Nagel™ 740948.10
This item is not returnable.
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Description
NucleoSpin™ RNA clean-up kits are recommended for the clean-up of total RNA from RNA preparations which contain inacceptable amounts of RT-PCR* inhibitors, often found in e.g., RNA prepared with phenol-chloroform based methods. It is further recommended for the isolation of RNA from small amounts of cultured cells whenever copurification of some genomic DNA is acceptable. For the isolation of RNA from cells and tissue with lowest DNA contamination of the isolated RNA we recommend DNase I containing NucleoSpin™ RNA kits (see ordering information). The kits allow purification of pure RNA with an A&subscript_begin;260/280&subscript_end; ratio generally exceeding 1.9 (measured in TE buffer (pH7.5)). NucleoSpin™ RNA clean-up kits are further recommended for the clean-up of RNA from enzymatic reactions like in vitro transcribed RNA, amplification reactions (e.g., ExpressArt™ Amino-Allyl-mRNA Amplification Kit, artus GmbH, Hamburg, Germany), biotinylated RNA or fluorescent (Cy dye) labelled RNA. The purified RNA is ready to use for applications like enzymatic labelling reactions (e.g., dye incorporation), reverse transcriptase-PCR (RT-PCR), and for DNA/RNA based chip hybridisations (e.g., MWG rat microarray, MWG, Ebersberg, Germany or Human Genome U133A Array, Affymetrix, USA). Integrity of purified RNA, originally isolated from e.g., eukaryotic cells, is examined by denaturing agarose gel electrophoresis: rRNA bands are sharp, with the 28S band being about twice as intense as the 18S band.- Silica membrane technology
- Typical recovery: 85% to 95%
- Sample material: ≤200μL phenol/chloroform extract or reaction mixture
- Elution volume: 40μL to 100μL
- Fragment size: >200b
- Binding capacity: 200μg
- Preparation time: 20min/6 preps
- Format: mini spin column
- RNA suitable for RT-PCR, Northern blotting, RNase protection assays, primer extension and array technology For RNA clean up of pre-purified RNA (e.g., Trizol); Reaction mixtures; RNA from small cell numbers; Amino-Allyl-mRNA; Biotinylated RNA.
Specifications
One-phase extraction | |
NucleoSpin™ RNA Clean-up Column | |
Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers | |
NucleoSpin™ RNA Clean-up Column | |
Reagent |
One-phase Extraction | |
For RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms | |
10 | |
1 Each |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
Supplemental information
- MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (denatured with 1%IPA/1%MEK, acc.2016/1867/EU)