Thermo Scientific™ Pierce™ Micro-Spin Columns

Description

Thermo Scientific™ Pierce Microcentrifuge Spin Cups and Spin Columns are convenient tools for manipulating small volumes of affinity supports for protein purification.

Additional Information and Online Ordering

These microcentrifuge spin cups and spin columns are convenient tools for manipulating small volumes of affinity supports (5 to 500μL) for immunoprecipitation (IP) and pull-down purification assays. Five styles of polypropylene columns and matching collection tubes offer a variety of options to suit particular types of experimental needs. Add the affinity resin and sample to one of the columns and use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity-purify more protein in less time.

Highlights:

• Efficient washing of samples means fewer washes are needed to remove contaminating proteins
• Efficient elution of samples means better recovery of immunoprecipitation and assay products
• Filter cups and columns eliminate contamination of eluted samples with affinity resin
• Filter cups and columns eliminate the need to decant supernatant from pelleted debris or resin
• Spin protocols drastically reduce the time required for small-scale affinity protocols
• Low protein-binding polypropylene column construction minimizes nonspecific binding

Spin Cups-Paper Filter with Collection Tubes (Part No. 69700):

• Column volume: 600μL
• Resin volume: 20 to 300μL
• Filter type: paper, 10μm pore size
• Cap type: collection tube cap fits onto inserted spin cup

Spin Cups-Cellulose Acetate Filter with Collection Tubes (Part No. 69702):

• Column volume: 800μL
• Resin volume: 20 to 400μL
• Filter type: cellulose acetate, 0.45μm pore size
• Cap type: collection tube cap fits onto inserted spin cup
• Spin ColumnsScrew Cap with Luer-Lok

Spin Columns-Screw Cap (Part No. 69705):

• Column Volume: 900μL
• Resin volume: 20 to 400μL
• Filter type: polyethylene filter, 10μm pore size; small and large frit options for different sample sizes
• Cap type: O-ring screw top caps; press-in bottom plugs
• Luer-Lok Adapters enable use with syringe-based kits

Spin Columns-Snap Cap with Collection Tubes (Part No. 69725):

• Column volume: 1000μL
• Resin volume: 20 to 500μL
• Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
• Cap type: snap cap on column (no cap on collection tube); press-on bottom caps

Micro-Spin Columns (Part No. 89879):

• Column volume: 400μL
• Resin volume: 5 to 100μL
• Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
• Cap type: O-ring screw top caps; press-on bottom caps

Microcentrifuge Tubes (Part No. 69715):

• 2mL capacity
• Dolphin-style bottom with attached snap cap
• Included and compatible with Part No. 69700

Microcentrifuge Tubes (Part No. 69720):

• 2mL capacity
• Short, conical bottom with attached snap cap
• Included and compatible with Part No. 69702