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Thermo Scientific™ Pierce™ SILAC Media and Sera
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These Thermo Scientific Pierce cell culture media and serum components are optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).
Highlights:
Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat
Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry
High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation
Specifikationer
Specifikationer
| Beskrivning | DMEM:F12 (1:1) Media for SILAC |
| Formulering | DMEM:F12 (1:1) media minus L-Lysine and L-Arginine for induced pluripotent cells |
| Produkttyp | DMEM:F12 |
| Tillräckligt för | Part of 1 SILAC experiment |
| Kvantitet | 500 mL |
| För användning med (applikation) | SILAC experiments (stable isotope labeling with amino acids in cell culture) |
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