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Thermo Scientific™ POROS™ MabCapture™ A Select Resin
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POROS™ MabCapture™ A Select Resin är ett nytt protein A-kromatografiharts med hög genomströmning till lägre priser, som kombinerar tidigare förbättringar av MabCapture A med vår egen Protein A-ligand, vilket möjliggör samma reningsprestanda med hög genomströmning till en betydande prissänkning jämfört med de flesta protein A-kromatografihartser. Dessa framsteg ger ett monoklonalt antikroppsreningsharts som uppvisar hög dynamisk bindningsprestanda vid låga linjära flödeshastigheter och bibehåller överlägsen bindningskapacitet vid höga linjära flödeshastigheter där andra kromatografimediaprodukter inte kan fungera.
- Maintain high dynamic binding capacity at linear flow rates over 700 cm/hr
- Design processes with greater flexibility (shorter bed heights, faster flow rates, or both) due to the improvements made to the antibody binding efficiency
- Obtain the highest dynamic binding capacity for antibodies vs. conventional agarose- or silica-based Protein A resins
- Purify for polyclonal IgG from human, rabbit, monkey, mouse, pig, dog, or cat serum
- Clean and sanitize with standard sodium hydroxide agents used for other chromatography products
- Recognize major increases to process productivity and reduce the downstream bottleneck
The Protein A molecule contains five high-affinity binding sites capable of binding to the Fc portion of antibodies. Compared to Protein G, Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs, and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A also binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype. The Protein A molecule also has greater stability than Protein G. Protein A is heat-stable and retains its native conformation when exposed to denaturing reagents such as 4M urea, 4M thiocyanate, and 6M guanidine hydrochloride.
The development of humanized monoclonal antibodies has continued to fuel the growth of the biotherapeutic market. Addressing the therapeutic demand for monoclonal antibodies has led to major improvements to the cell-based expression systems that are used to manufacture these proteins. >
Consequently, these improvements have moved the processing bottleneck from upstream cell expression to downstream purification. Protein A chromatography, which is used as the primary capture chromatography step in most antibody process platforms, is generally regarded as the major process bottleneck due to the slow flow rates and long residence times required for the effective operation of conventional Protein A chromatography resin. POROS MabCapture Select chromatography resin offers the capacity, efficiency, and flexibility needed to make improvements to the downstream purification platform, resulting in major increases to process productivity and antibody manufacturing
Specifikationer
Specifikationer
| Typ | A Select Resin |
| Standardkomponenter | Protein A |
| För användning med (applikation) | IgG Purification |
| Volym (metrisk) | 15 ml |
| Innehåll och lagring | Store in refrigerator (2–8°C). |
| Produktlinje | POROS |
| Formatera | Poros Bead |
| Stationär fas | Protein A |
| Reningsmål | IgG Antibodies, Ig Antibodies |
| Frakt skick | Wet Ice |
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