THRA/THRB Monoclonal Antibody (C3), Invitrogen™

Description

MA1-215 detects thyroid hormone receptor (TR) from human tissues. This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1. MA1-215 has been successfully used in Western blot, IF, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 55 and a 52 kDa protein representing recombinant human TR beta-1 expressed in E. coli. MA1-215 immunoprecipitates 52 kDa and 55 kDa proteins, representing TR beta-1 and a 48 kDa protein representing TR alpha-1 expressed in E. coli. The MA1-215 immunogen is a purified fragment of human TR beta-1 corresponding to amino acid residues 201-456. This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1.

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene.