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General Western Blot Reagents

General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 51 results
1

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Promotions are available
Content And Storage Upon receipt store at room temperature. Product shipped at ambient temperature.
Form Liquid
Product Line Restore™
2

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Promotions are available
Content And Storage Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form Liquid
Product Line Restore™
3

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

EDGE
4

Invitrogen™ iBind™ Flex Solution Kit

Detection kit for use with iBind™ Flex Western System

Promotions are available
EDGE
No. of Reactions 10 midi blots or 20 mini blots
Product Type Flex Solution Kit
For Use With (Equipment) iBind Flex Western Device
Membrane Compatibility PVDF, Nitrocellulose
Product Line iBind™
Detection Method Colorimetric, Chromogenic, Chemiluminescence
5

Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Promotions are available
Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility PVDF, Nitrocellulose
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Product Line Invitrogen™
6

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Promotions are available
Product Type Transfer Buffer
7

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled

Promotions are available
EDGE
8

Invitrogen™ Bolt™ Antioxidant

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Promotions are available
EDGE
9

Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels

Promotions are available
10

Invitrogen™ Incubation Tray, 10 x 14 cm

Enables convenient and even staining of E-PAGE™ blots

11

Invitrogen™ XCell II™ Blot Module

Easy transfer of proteins or nucleic acids from mini-gels to membranes

Promotions are available
12

Invitrogen™ iBind™ Flex Fluorescent Detection (FD) Solution Kit

Detection kit for use with iBind™ Flex Western System

Promotions are available
13

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)

Optimized, easy-to-use blocker and primary antibody diluent system

14

Invitrogen™ WesternBreeze™ Wash Solution (16X)

Optimized solution of concentrated buffered saline containing detergent

15

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)