Protein Purification Reagents and Kits
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Protein Purification Reagents and Kits
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Filtered Search Results
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Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Sartorius Sartoclear Dynamics™ Lab V Filtration Kit
Sartorius Sartoclear Dynamics™ Lab V Kits designed for the clarification and sterile filtration of mammalian cell culture with diatomaceous earth as filter aid.
Product Type | Vacuum Filtration Units |
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Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
Type | Agarose |
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Stationary Phase | Ni-NTA |
Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
Form | Suspension |
Column Type | Affinity Column |
Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Buffer | Lysis Buffer |
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Shipping Condition | Ambient |
Form | Liquid |
Product Type | Nuclear and Cytoplasmic Extraction Reagent |
Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
Product Line | NE-PER™ |
Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Agarose |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | 20 mg His-Tagged GFP Binding per mL Resin |
Product Line | HisPur™ |
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Stationary Phase | Proprietary Ligand |
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Column Type | Proprietary Resin, Affinity |
Purification Target | Detergent Removal |
Thermo Scientific™ SulfoLink™ Coupling Resin
Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Stationary Phase | Haloacetyl |
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Type | Resin |
Content And Storage | Upon receipt store product in the dark at 4°C. Product is shipped at ambient temperature. |
Format | Bottle |
Column Type | Affinity, Agarose Resin |
Sufficient For | 1 mg Peptide or 10 mg Antibody per mL of Resin |
Product Line | SulfoLink™ |
Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.