Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.

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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Sample Type	RNA
Reaction Speed	Standard
Product Line	AgPath-ID™, Ambion™

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System
Reaction Speed	Standard
Product Line	AgPath-ID™, Ambion™

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

Pricing and Availability
Specifications

GC-Rich PCR Performance	Low
Format	Tube(s)
Internal Probe Modification	TAMRA™ Quencher (3'), VIC™ (5')
Product Type	Exogenous Internal Positive Control Reagent
Reaction Speed	Standard
Green Features	Sustainable packaging
PCR Method	qPCR
Product Line	TaqMan™

Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

Provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis.

Pricing and Availability

Applied Biosystems™ TaqMan™ Reagents Starter Kit

TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.

Pricing and Availability

Applied Biosystems™ TaqMan™ Fast Reagent Starter Kit

Designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5' nuclease assay, in fast thermal cycling mode.

Pricing and Availability

Thermo Scientific™ SureTect™ Brettanomyces spp. and Saccharomyces cerevisiae Multiplex PCR Assay

Rapidly detect and differentiate Brettanomyces spp. and S. cerevisiae in concentrate, semi-finished products, and environmental samples with the Thermo Scientific™ SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay.