Test
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Product Type | Transfer Buffer |
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Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restore™ |
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restore™ |
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Made with high-purity reagents and are strictly quality controlled
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Detection kit for use with iBind™ Flex Western System
No. of Reactions | 10 midi blots or 20 mini blots |
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Product Type | Flex Solution Kit |
For Use With (Equipment) | iBind Flex Western Device |
Membrane Compatibility | PVDF, Nitrocellulose |
Product Line | iBind™ |
Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Shipping Condition | Dry Ice |
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Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
Product Type | Protein Labeling Reagent |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Membrane Compatibility | Nitrocellulose, PVDF |
Green Features | Less hazardous |
Shelf Life | At least 2 years from receipt |
Run Time | 10 minutes |
For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Product Line | Invitrogen™ |
Optimized, easy-to-use blocker and primary antibody diluent system
Optimized solution of concentrated buffered saline containing detergent
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
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Form | Liquid |
Product Type | Western Blot Enhancer |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence, Fluorescence, Colorimetric |