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Cytiva Glutathione Sepharose™ 4B Media
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Cytiva Glutathione Sepharose™ 4 Fast Flow Media
Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.
Particle Size | 90 μm |
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Ligand Type | Glutathione and 10-carbon linker arm |
Flow Rate | <450 cm/hr. |
Type | Chromatography Media |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Matrix | Highly cross-linked 4% agarose |
Kit Contents | 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size |
Description | Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed. |
Max. Operating Pressure | base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
pH Range | 3 to 12 |
Binding Capacity | >10 mg/mL |
Support Type | 4% Highly Cross-linked Agarose |
Content And Storage | 4°C to 30°C, 20% Ethanol |
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Diameter (English) Inner | 0.27 in. |
Bed Volume | 1 mL |
Description | GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins. |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 7 mm |
Material | Polypropylene |
For Use With (Application) | For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract |
Particle Size | 90 μm |
Ligand Type | Glutathione and 10 carbon linker arm |
Flow Rate | <4 mL/min. |
Type | Pre-packed Column |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
Support Type | 4% Highly Cross-linked Agarose |
Cytiva GST Gene Fusion System pGEX Vectors
Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST
Type | pGEX Vector |
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Quantity | 25 μg |
For Use With (Application) | Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schisto soma japonicum GST |
Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm
For fast and simple purification of GST-tagged proteins
Content And Storage | 4°C to 30°C, 20% Ethanol |
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Diameter (English) Inner | 0.62 in. |
Bed Volume | 5 mL |
Description | GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. |
pH Range | 4 to 13 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
For Use With (Application) | GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function |
Particle Size | 45 to 165 μm |
Ligand Type | Glutathione |
Flow Rate | <20 mL/min. |
Type | Pre-packed Column |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
Support Type | 4% Highly Cross-linked Agarose |
Cytiva GSTrap™ HP Columns
Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.
Particle Size | 34 μm |
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Ligand Type | Glutathione |
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Description | GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
For Use With (Application) | Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins |
Support Type | 4% Highly Cross-linked Agarose |
Content And Storage | 4°C to 30°C, 20% Ethanol |
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Diameter (English) Inner | 0.62 in. |
Bed Volume | 5 mL |
Description | GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins. |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
For Use With (Application) | For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract |
Particle Size | 90 μm |
Ligand Type | Glutathione and 10 carbon linker arm |
Flow Rate | <20 mL/min. |
Type | Pre-packed Column |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
Support Type | 4% Highly Cross-linked Agarose |
Cytiva Glutathione Sepharose™ High Performance Media
For high resolution purification of Glutathione S transferase (GST) tagged proteins
Particle Size | 34 μm |
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Ligand Type | Glutathione |
Flow Rate | <600 cm/hr. |
Type | Chromatography Media |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Matrix | Highly crossed-linked agarose, 6% |
Description | Glutathione Sepharose High Performance is an affinity resin recommended for purifying glutathione S-transferase (GST)-tagged proteins when high resolution is the priority. |
pH Range | 3 to 12 |
Binding Capacity | >Approx. 10 mg GST-tagged protein/mL resin |
Support Type | 6% Highly Cross-linked Agarose |