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Invitrogen™ MEGAclear™ Transcription Clean-Up Kit

Quickly cleanup large-scale transcription reactions

Brand:  Invitrogen™ AM1908


Product Code. 10600226

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Description

Description

MEGAclear Kit is designed for quick cleanup of any large-scale transcription reaction. MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.
  • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
    High recovery from 1ng to 500μg of input
    Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
    Quick protocol—purify RNA in less than 30 minutes
  • Using the MEGAclear Kit: Protocol is quick and easy. Transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
Tube
mRNA, Synthetic mRNA
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Not High-throughput Compatible (Manual)
Ambion™, MEGAclear™
20 Preps
Room Temperature
500 μg
Purified Synthetic mRNA, Purified mRNA
Silica Spin Column
mRNA, Synthetic mRNA
Vacuum Manifold, Centrifuge
20 Preps
mRNA purification kit, Purification kit
30 min
1 ng to 500 μg of dsRNA>200b or ssRA>100bp
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Safety and Handling

Safety and Handling

Product Identifier
  • MEGAclear Transcription Clean-Up Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Skin corrosion/irritation Category 1 C

Hazard Statement
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P330-Rinse mouth.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : 2-Mercaptoethanol

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.