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Invitrogen™ PureLink™ RNA Mini Kit
Provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol
Brand: Invitrogen™ 12183018A
2362.75 SEK valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
- Obtain high-yield, high-integrity RNA
- Rapid, non-hazardous RNA purification capabilities
- Ready-to-use RNA for many downstream applications
Specifications
| • 125 mL Lysis Buffer; store at room temperature • 50 mL Wash Buffer I; room temperature • 15 mL Wash Buffer II; room temperature • 15.5 mL RNase-free Water; room temperature • 50 PureLink Spin Cartridges with Collection Tubes; room temperature • 50 Collection Tubes; room temperature • 50 Recovery Tubes; room temperature |
|
| 30–300 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Mini | |
| Bacteria: ≤109 cells Blood: ≤0.2 mL Cells: ≤5 x 107 Plant: ≤250 mg Tissue: ≤200 mg Yeast: ≤5 x 108 cells Enzymatic reactions: ≤1.2 mL |
| Silica Spin Column | |
| Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions (DNase digestions, RNA labeling/cleanup preps, IVT) | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Room Temperature | |
| Up to 350 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- PURELINK RNA MINI KIT
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 1 C
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : DyLight Reactive Dyes, Thiocyanic acid