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Invitrogen™ RNAqueous™ Total RNA Isolation Kit

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

Brand:  Invitrogen™ AM1912

4222.50 SEK valid until 2024-12-20
Use promo code "21651" to get your promotional price.


Product Code. 10075994

  • 5630.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)

Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
50–120 μL
Total RNA
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Room Temperature
Spin Column (Glass Fiber Filter)
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Not High-throughput Compatible (Manual)
50 Preps
Bacteria: ≤109 cells
Cells: ≤107
Tissue: ≤75 mg
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • RNAqueous Total RNA Isolation Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Carcinogenicity Category 1B
  • Serious eye damage/eye irritation Category 2
  • Germ cell mutagenicity Category 2
  • Reproductive toxicity Category 1B
  • Skin corrosion/irritation Category 1 B
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H341-Suspected of causing genetic defects.
  • H350-May cause cancer.
  • H360-May damage fertility or the unborn child.
  • H371-May cause damage to organs.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Lithium chloride, Formamide, Formaldehyde, Methanol, 2-Mercaptoethanol

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For Research Use Only. Not for use in diagnostic procedures.