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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
Brand: Molecular Probes™ A23210
This item is not returnable.
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Description
In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Specifications
Green | |
Fluorescence | |
490/525 | |
Flow Cytometry | |
Alexa Fluor™ 488 | |
APO-BrdU™ | |
Mouse | |
Alexa Fluor Dyes |
Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL). Store as directed. |
|
Tube | |
488 | |
Fluorescence Microscope, Flow Cytometer | |
TUNEL Assay Kit | |
60 Assays | |
BrdU |
For Research Use Only. Not for use in diagnostic procedures.