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BD Annexin V
For flow cytometric analysis of cells that are undergoing apoptosis
Brand: BD 556416
Alert:
Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing
Description
Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of cytoplasm and nucleus, and internucleosomal cleavage of DNA. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from inner to outer leaflet of plasma membrane, thereby exposing PS to external cellular environment
- 35-36 kDa Ca2+ dependent phospholipid-binding protein that has high affinity for PS
- Binds to cells with exposed PS
- May be conjugated to fluorochromes, such as fluorescein isothiocyanate (FITC), to serve as sensitive probe
- Typically used in conjunction with vital dye such as 7-Amino-Actinomycin
- Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide
- Format: Purified
Flow cytometry, Blocking
Specifications
| 0.5mg/mL | |
| Aqueous Buffered solution containing ≤0.09% Sodium Azide | |
| Annexin V | |
| -80°C | |
| Solution |
| Blocking Agent, Flow Cytometry | |
| 35 to 36 | |
| 0.1 mg | |
| Recombinant |
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