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BD Cell Enrichment Cocktail
For negative selection of untouched memory CD4+ helper T cells from peripheral blood
Brand: BD 558521
Includes:
Human Memory CD4+ T Cell Enrichment Cocktail, 5.0mL:
- Biotin Mouse Anti-human CD8, clone SK1
- Biotin Mouse Anti-human CD11b, clone ICRF44
- Biotin Mouse Anti-human CD16, clone 3G8
- Biotin Mouse Anti-human CD19, clone HIB19
- Biotin Mouse Anti-human CD36, clone CB38
- Biotin Mouse Anti-human CD41a, clone HIP8
- Biotin Mouse Anti-human CD45RA, clone HI100
- Biotin Mouse Anti-human CD56, clone B159
- Biotin Mouse Anti-human CD123, clone 9F5
- Biotin Mouse Anti-human CD235a (Glycophorin A), clone GA-R2 (HIR2)
- Biotin Mouse Anti-human γδ TCR, clone B1
- In aqueous buffered solution containing 0.09% Sodium Azide
BD IMag Streptavidin Particles Plus - DM, 7.5mL:
- In aqueous buffered solution containing BSA™ and 0.09% Sodium Azide
Description
Contains biotinylated monoclonal antibodies that recognize antigens expressed on naive T cells, CD8+ T cells, B cells, NK cells, Γ/δ T cells, monocytes, DCs, granulocytes, platelets, and erythroid cells
Negative selection is performed to enrich for unlabeled memory cells. Labeled cells migrate towards magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off and retained (enriched fraction)
- Contains biotinylated monoclonal antibodies that recognize antigens expressed on naive T cells, CD8+ T cells, B cells, NK cells, γ/δ T cells, monocytes, DCs, granulocytes, platelets, and erythroid cells
- Avoids inadvertent activation of enriched memory helper T cells by using reagents that do not directly bind to those T cells
- Simultaneously stains erythrocytes, platelets, and most leukocytes
Flow cytometry
Specifications
| In aqueous buffered solution containing BSA™ 0.09% sodium azide at 4°C | |
| Flow cytometer | |
| Naive CD4+ T Cell Enrichment Cocktail |
| For negative selection of untouched memory CD4+ helper T cells from peripheral blood, magnetic cell separation | |
| Biotin mouse Anti-Human CD8, clone SK1, CD11b, clone ICRF44, CD16, clone 3G8, CD19, clone HIB19, CD36, clone CB38, CD41a, clone HIP8, CD45RA, clone HI100, CD56, clone B159, CD123, clone 9F5, CD235a (Glycophorin A), clone GA-R2 (HIR2), gd TCR, clone B1 | |
| 5 mL |
Research use only