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BD HeLa + Anisomycin Lysate
Convenient to use and provided in denatured format allowing for immediate loading onto SDS-PAGE gel
Brand: BD 611692
Additional Details : Weight : 0.01100kg
Includes: Human Epitheloid Cervical Carcinoma
Description
- Useful control for western blotting application
- Source that can be rich in endogenously expressed proteins
- Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)
Specifications
Western Blot | |
HeLa (Human Epitheloid Carcinoma) cells, were grown to 80 to 90% confluency, then treated with 25μg/mL Anisomycin, an antibiotic and Protein synthesis inhibitor, for 15 min. at 37°C | |
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol | |
1 mg/mL |
Epitheloid Cervical Carcinoma | |
500 μg | |
Human | |
-20°C |