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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Brand: Applied Biosystems™ 4337458
Description
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
- Improve the quality of your results for a wide range of sequencing applications
- Optimized for long read lengths
- Better dye mobility characteristics
- Improved performance reading through GT-rich regions
- Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
- Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
Specifications
• 10 x 20 mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix • 4 tubes M13 (-21) Primer • 4 tubes pREF-BDT™ Control DNA • 1 x 233 mL tube of 5X Sequencing Buffer (may be stored at 4°C) Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles. |
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SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers | |
BigDye™ Terminator | |
25,000 Reactions | |
BAC DNA, Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA |
12-well Strips, 8-well Strip, 96- or 384-well Plate | |
Standard Sequencing | |
Sequencing Buffer | |
Fluorescent Dye Terminator Sequencing |
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For Research Use Only. Not for use in diagnostic procedures.