missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Macherey-Nagel™ Carrier RNA
Carrier-RNA is added to the sample lysate (20 ng/sample) when using compatible NucleoSpin kits (RNA Virus, RNA XS) for optimal performance and highest sensitivity in downstream applications.
Brand: Macherey-Nagel™ 740514
This item is not returnable.
View return policy
Description
For use with RNA isolation using various NucleoSpin and NucleoMag kits: Virus, XS (extra small samples)
- (poly(-A) RNA: poly(A) potassium salt—prepared from ADP with polynucleotide phosphorylase)
- To prepare working solution, dissolve freeze dried Carrier-RNA in lysis buffer (e.g. RA1, RAV1)
- Add to sample lysate (20 ng/sample)
- Such small amounts typically do not interfere with subsequent RT-PCR, even in oligo-dT primed reverse transcriptions
- The small amount of Carrier RNA transfered into a reverse transcription reaction does not significantly influence the outcome of the reaction, due to the large excess of oligo-dT primer
- The benefit of adding Carrier RNA depends on sample type, amount, and kind of downstream RNA analysis
- Carrier RNA should be omitted when, subsequent to RNA isolation, a poly-A RNA isolation / RNA sequencing is performed
Specifications
| 26763-19-6 | |
| Colorless | |
| poly(-A) RNA: poly(A) potassium salt | |
| RNA isolation for downstream applications | |
| 0.3 mg | |
| Lyophilized |
| 90 to <100% | |
| Refrigerated (4 °C) | |
| NucleoSpin Virus and XS kits | |
| Odorless | |
| Prepared from ADP with polynucleotide phosphorylase |