missing translation for 'onlineSavingsMsg'
Learn More

CD3 Monoclonal Antibody (17A2), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

520.00 SEK - 3225.00 SEK

Specifications

Antigen CD3
Clone 17A2
Concentration 0.5 mg/mL
Applications Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry (Frozen)
Classification Monoclonal
View More Specs

Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15537416
View Documents Promotion Details
Invitrogen
14-0032-81
50 μg
520.00 SEK
50µg
Please to purchase this item. Need a web account? Register with us today!
15547416
View Documents Promotion Details
Invitrogen
14-0032-82
100 μg
924.00 SEK
100µg
Please to purchase this item. Need a web account? Register with us today!
15557416
View Documents Promotion Details
Invitrogen
14-0032-85
500 μg
1926.00 SEK
500µg
Please to purchase this item. Need a web account? Register with us today!
15226737
View Documents Promotion Details
Invitrogen
14-0032-86
1 mg
3225.00 SEK
1mg
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: The 17A2 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of mouse T cells. (Please use Functional Grade purified 17A2, cat. 16-0032, in functional assays). Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.
Specifications

Specifications

CD3
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen d
Cd247, Cd3d, CD3E, CD3g
Primary
4° C
17A2
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry (Frozen)
Unconjugated
Rat
Mouse
P04235, P11942, P22646, P24161
12500, 12501, 12502, 12503
IgG2b κ
Affinity chromatography
Antibody
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.