CD38 Monoclonal Antibody (5C5C3), Invitrogen™

Description

By Western blot, MA1-182 detects a band at ∽45kD, which likely represents the glycosylated form of CD38. Different lysis buffers can be used to prepare lysates for Western blot with MA1-182, but depending on the level of CD38 expression, it may be necessary to use a membrane extraction buffer (e.g. Mem-PER Plus Membrane Protein Extraction Kit, Product # 89842).

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.