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CD45 Monoclonal Antibody (2D1), Super Bright™ 645, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

1750.00 SEK - 4245.00 SEK

Specifications

Antigen CD45
Clone 2D1
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15805703
View Documents
Invitrogen
64-9459-41
25 Tests This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
15815703
View Documents
Invitrogen
64-9459-42
100 Tests
4245.00 SEK
Pack of 100
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Description

Description

Description: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (∽100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors. Applications Reported: This HTA125 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HTA125 antibody has been pre-diluted and tested by flow cytometric analysis of cells transfected with human TLR4. This may be used at 5 μL (0.5 μg) per test.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Specifications

Specifications

CD45
5 μL/Test
Monoclonal
Liquid
RUO
P08575
PTPRC
Primary
4° C, store in dark, DO NOT FREEZE!
PTPRC
2D1
Flow Cytometry
Super Bright 645
Mouse
Human
5788
IgG1 κ
Affinity chromatography
Antibody
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CD45 Monoclonal Antibody (2D1), Super Bright™ 645, eBioscience™, Invitrogen™

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