CD64 Monoclonal Antibody (X54-5/7.1), APC, eBioscience™, Invitrogen™

Description

Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. Alloform a is present in the following mouse strains: C57BL/6, BALB/c, DBA/2, and alloform b in strains: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ. In addition this antibody has been reported positive in strains: AKR, ALR, BUB, C58, CE, HRS,MRL, MON, NZB, NZO, NZW, PL, SJL, ST and SWR. This clone X54-5/7.1 will not recognize the allelic variant d, present in NOD/Lt mice. This antibody has been reported to stain RAW264.7 cell line. Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.