Cdc27 Monoclonal Antibody (5C12), Invitrogen™

Description

MA5-15655 targets CDC27 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15655 immunogen is purified recombinant fragment of human CDC27 expressed in E. Coli. <br>. MA5-15655 detects CDC27 which has a predicted molecular weight of approximately 91kDa.

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.