CXCL6 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Mutine LIX (CXCL6). Anti-Murine LIX (CXCL6)-specific antibody was purified by affinity chromatography employing an immobilized Murine LIX (CXCL6) matrix. Sandwich ELISA: To detect Murine LIX (CXCL6) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine LIX (CXCL6) (500-P146Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine LIX (CXCL6). Western Blot: To detect Murine LIX (CXCL6) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIX (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).