Thermo Scientific™ DNAPac™ RP Columns

Description

Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs.

DNAPac RP reversed phase (RP) column is based on spherical wide-pore 4 μm polymer resin specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is highly stable to extreme pH (0-14), and temperature (5-110°C) and fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of small to large nucleic acids with low carry-over. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments:

• Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both oligonucleotides and double-stranded nucleic acids
• High resolution and high throughput
• Excellent MS compatibility
• Wide operating pH range (0-14) and high temperature stability (up to 110°C)
• Biocompatible hardware to eliminate analyte absorption