Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Beschreibung

Thermo Scientific DreamTaq Hot Start PCR Master Mix, available in colorless and green formats, is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq Buffer, magnesium, and dNTPs.

The master mix allows easy reaction setup and fewer pipetting steps, with only primers, template, and water needing to be added. The DreamTaq Hot Start Green PCR Master Mix includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity through:

• Better reaction outcomes
  • Higher yield of target amplicons from low template amounts
  • Increased reaction specificity due to reliable hot-start technology
  • Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
  • Room temperature reaction setup
  • Minimized optimization of Mg²⁺ concentration and of primer annealing temperatures due to optimized reaction buffer
  • Simplified workflow and minimized pipetting steps with 2X master mix format
  • Direct loading of PCR products on a gel with green format

Applications

• Generation of PCR products for TA cloning
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR