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Epo Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

2105.00 SEK - 18720.00 SEK

Specifications

Antigen Epo
Concentration 0.1-1.0 mg/mL
Applications ELISA, Western Blot
Classification Polyclonal
Conjugate Unconjugated
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
17855373
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Invitrogen
500-P318-50UG
50μg
2105.00 SEK
50µg
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17865793
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Invitrogen
500-P318-100UG
100 μg
2700.00 SEK
100µg
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17845333
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Invitrogen
500-P318-1MG
1 mg
18720.00 SEK
1mg
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Description

Description

AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix. Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EPO (500-P318Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EPO. Western Blot: To detect Human EPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
Specifications

Specifications

Epo
ELISA, Western Blot
Unconjugated
Rabbit
Human
P01588
2056
CHO cells-derived Recombinant Human EPO
Antigen affinity chromatography
EPO
0.1-1.0 mg/mL
Polyclonal
Lyophilized
RUO
PBS with no preservative
EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
EPO
Primary
-20°C
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