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Thermo Scientific™ DraI (10 U/μL)

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

Brand: Thermo Scientific™ ER0221

485.28 SEK valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Product Code. 10679880

674.00 SEK / 1500 units

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This item is not returnable. View return policy

Description

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

5'...T T T▵A A A...3'

3'...A A A▵T T T...5'

Conditions for 100% Activity:

1X Buffer Tango™:
33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
Incubate at 37°C

Storage Buffer:

DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

Dam: never overlaps — no effect
Dcm: never overlaps — no effect
CpG: never overlaps — no effect
EcoKI: may overlap — blocked
EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Specifications

Concentration	10 U/μL
Enzyme	Dra I
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	10X Buffer Tango
Optimal Reaction Temperature	37°C
Quantity	1,500 units
Research Category	Traditional Cloning

Product Suggestions Documents

Documents

Product Information

Manuals

Digestion of agarose-embedded DNA Version: Rev B (18 March 2016) File size: 96.3 kB

User Guide: DraI, 10 U/uL, 1500U Version: Jan. 2015 File size: 70.9 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2814736	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0221
Lot Number2877539	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0221
Lot Number2932466	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberER0221
Lot Number2978071	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberER0221
Lot Number3030341	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberER0221

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Thermo Scientific™ DraI (10 U/μL) > 10U/μL, 1500U

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