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  5. Thermo Scientific™ X-Gluc

Thermo Scientific™ X-Gluc

Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.

1112.00 SEK - 3305.00 SEK

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Specifications

Detection Method Colorimetric
Product Type X-Gluc
Substrate Properties Chemical Substrate
Substrate Type Beta-Glucuronidase (GUS) Substrate
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10509140
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Thermo Scientific™
R0851 		0.1 g
1112.00 SEK
0.10g
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10519140
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Thermo Scientific™
R0852 		0.5 g
3305.00 SEK
500mg
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Description

Description

X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Recommended for:

  • Detection of GUS expression in plant cells and tissues (1)
  • Detection of infections caused by E.coli
  • Detection of bacterial contamination in food and water samples

Note:

Preparation of a 0.1M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

Specifications

Specifications

Colorimetric
X-Gluc
Chemical Substrate
Beta-Glucuronidase (GUS) Substrate
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For Research Use Only. Not for use in diagnostic procedures.

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