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Thermo Scientific™ Fluorescein-D22 Coactivator Peptide, 100 μM
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.
Brand: Thermo Scientific™ PV4387
Additional Details : Weight : 0.50000kg
Description
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.
How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).
Specifications
| ≥95% by HPLC | |
| 100 μm | |
| Fluorescein-D22 Coactivator Peptide, 1mL, 100μM Concentration, 2499Da Molecular Weight, –5 to –30°C Storage Condition, Used for Pharma and Biopharma, Target and Lead Identification and Validation | |
| Classic Dyes | |
| Fluorescein - D22 Coactivator Peptide | |
| For research use only. Not for use in diagnostic procedures | |
| Dry Ice |
| Fluorescein - D22 Coactivator Peptide | |
| LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers. Store peptides at -20°C. | |
| Pharma and biopharma, target and lead identification and validation | |
| 2499Da | |
| 1 mL | |
| Fluorescein- LPYEGSLLLKLLRAPVEEV | |
| 10mM Na2CO3 (pH 11.0) |
For Research Use Only. Not for use in diagnostic procedures.