Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Description

• Glow assay kinetics provides a window during which measurements may be performed, facilitating HTS applications where assay automation is used
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
• Assay can be completed in about one hour, providing fast assay turnaround

Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Versatile
The Galacto-Star system includes Galacton-Star chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal
Light emission from the Galacto-Star assay reaches maximum in 60 to 90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.

Specifications

• Assay Type: β-Gal (lacZ) Assay
• Compatible Cells: Mammalian Cells
• Detection Method: Chemiluminescent
• For Use With Equipment: Luminometer (Microplate)
• Form: Liquid
• Format: 96-well plate
• High Throughput Compatibility: High Throughput-Compatible
• Readout: End Point
• Sample Type (Specific): Cell Cultures
• Substrate: Galacton-Star
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase