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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Brand: Invitrogen™ A21175

Product Code. 15338484

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Alert:

For research use only. Not for use in diagnostic procedures.

Includes:

CRISPR CD4 Nuclease Vector, linearized
10X Oligonucleotide Annealing Buffer
DNase/RNase-free Water
5X Ligation Buffer
T4 DNA Ligase
U6 Forward Sequencing Primer
Control Double-stranded Oligonucleotide

This item is not returnable. View return policy

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter.

GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).

GeneArt™ CRISPR Nuclease Vector system offers:

• Easy-to-design genome engineering system

• Affordable, ready-to-use cloning vectors

• Enrichment for hard to transfect or difficult to modify cell

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

Cloning Method	Restriction Enzyme/MCS
Detection Method	Primer-probe
Format	Kit
Technique	CRISPR-Cas9
Product Type	Crispr Nuclease Vector With CD4 Enrichment Kit
Protein Tag	CD4 fusion
Sufficient For	10 Reactions
No. of Reactions	10 Reactions
Starting Material	Oligos (DNA)

Content And Storage	Contains: • CRISPR CD4 Nuclease Vector, linearized • 10X Oligonucleotide Annealing Buffer • DNase/RNase-free Water • 5X Ligation Buffer • T4 DNA Ligase • U6 Forward Sequencing Primer • Control Double-stranded Oligonucleotide Store in freezer (-5 to -30°C).
Form	Liquid
Reaction Speed	Fast
Product Line	GeneArt™
Promoter	U6, CMV
Reporter Gene	CD4
For Use With (Application)	Gene Expression, Cloning
Overhang	3' overhangs

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For Research Use Only. Not for use in diagnostic procedures.