Invitrogen™ GeneBLAzer™ ADORA2A CHO-K1 DA Assay Kit

Description

This cell line is available as dividing cells by requesting a quote for K1245 from dividingcells@invitrogen.com

GeneBLAzer™ ADORA2A CHO-K1 DA (Division Arrested) cells and ADORA2A-CRE-bla CHO-K1 cells contain the human Adenosine A2a (ADORA2A) receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ADORA2A CHO-K1 DA cells and ADORA2A-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC₅₀ concentrations of 5’-adenosine (NECA); (Figure 1). In addition, ADORA2A-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The Adenosine A2a Receptor (ADORA2A) has been shown to have many roles throughout the body. ADORA2A is involved in pain and inflammation regulation, inhibition of platelet aggregation, blood pressure regulation and contributes to ischemic brain damage. Selective antagonists of the ADORA2A pathway are being tested as adjuvants to dopaminergic drugs to treat Parkinson’s disease and schizophrenia.


Storage:

GeneBLAzer™ ADORA2A CHO-K1 DA Cells and ADORA2A-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ ADORA2A CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:

ADORA2A CHO-K1 DA cells

LiveBLAzer™-FRET B/G Loading Kit, 70μg

Solution D, 1ml

2 protocols

Certificate of Analysis
GeneBLAzer™ ADORA2A CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

ADORA2A CHO-K1 DA cells

1 protocol

Certificate of Analysis
GeneBLAzer™ ADORA2A-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

ADORA2A-CRE-bla CHO-K1 cells

1 protocol

Certificate of Analysis