Thermo Scientific™ GeneBLAzer™ CRHR1 CHO-K1 DA Assay Kit
NM_004382.2
GeneBLAzer™ CRHR1 CHO-K1 DA (Division Arrested) cells and CRHR1-CRE-bla CHO-K1 cells contain the human Corticotropin Releasing Factor 1 (CRHR1) receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1367
Product Code.10246893
8600.00 SEK
/ Each
Please sign in to purchase this item. Need a web account? Register with us today!
Division Arrested (DA) cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both CRHR1 CHO-K1 DA cells and CRHR1-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of corticotropin releasing factor (CRF), (Figure 1). In addition, CRHR1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Corticotropin releasing factor is a 41-amino acid peptide that plays a role in the integration of autonomic, neuroendocrine, and behavioral responses to stress. These effects are mediated through two receptor families, CRHR1 and CRHR2. While CRF was originally isolated from the hypothalamus, where it was shown to be the primary neuroregulator mediating the hypothalamic-pituitary-adrenocortical stress axis, it has since been found to be widely distributed outside the hypothalamus throughout the central nervous system. Presently, there are five distinct targets for CRF with unique pharmacology and localization. These have been placed into three distinct classes, two of which are the G-protein-coupled receptors CRF1 (CRHR1) and CRF2 (CRHR2). Three functional splice variants have been identified for the mammalian CRHR2 receptor, although pharmacological characterization of these splice variants has revealed no major differences between them.
GeneBLAzer™ CRHR1 CHO-K1 DA Cells and CRHR1-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.
Contents:
GeneBLAzer™ CRHR1 CHO-K1 DA Assay Kit Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: •CRHR1 CHO-K1 DA cells •LiveBLAzer™-FRET B/G Loading Kit, 70μg •Solution D, 1ml •2 protocols •Certificate of Analysis
GeneBLAzer™ CRHR1 CHO-K1 DA cells Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: •CRHR1 CHO-K1 DA cells •1 protocol •Certificate of Analysis
GeneBLAzer™ CRHR1-CRE-bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: •CRHR1-CRE-bla CHO-K1 cells •1 protocol •Certificate of Analysis
Customers who viewed this item also viewed
This information does not imply a recommendation or representation of any kind and any action taken upon the information provided is strictly at your own risk. The products shown have not been qualified by Fisher Scientific for any particular purpose, application or use and it is the responsibility of the purchaser to ensure that the performance of the product is suitable for the purchasers specific use or intended application.
