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Thermo Scientific™ GeneBLAzer™ PPARδ-UAS-bla HEK 293T Cells

PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

Brand:  Thermo Scientific™ K1690

Product Code. 10155793

  • 98300.00 SEK / Each
Estimated Shipment: 28-01-2025
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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of L-165041. In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Estriol, Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Cell-based beta lactamase reporter gene
PPAR delta-UAS-bla HEK 293T cells (K1244)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen), Liquid Nitrogen
Primary Agonist: 17-β-estradiol
Human
PPAR delta-UAS-bla HEK 293T cells (K1244)
17-β-estradiol
2 x 106 Cells
BLA (Beta-Lactamase)
Dividing Cells
RU486+1 nM E2, 4-OH-tam+1 nM E2, 4-OH-tamoxifen, Tamoxifen, Tam+1 nM E2, RU486
Dividing Cells
Fluorescence, Fluorescent
Nuclear Receptors
Nuclear Receptors
2 x 106
GeneBLAzer™
HEK 293T Cells
PPAR delta, NR1C2, PPARD
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For Research Use Only. Not for use in diagnostic procedures.